rtpcr.co.uk - the source for PCR information

RT PCR HOMERT PCR Informationrt pcr productsContact rtpcr.co.uk
pcr techniques

www.rtpcr.co.uk
 
  • 1. RT-PCR
  • 2. PCR-Detection & Monitoring
  • 3. Competit. & NonCompet. PCR
  • 4. PCR Protocols
  • 5. Quantification
  • 6. References
 

INFORMATION PAGE - RT PCR REFERENCES

 

References

1.Orlando C, Pinzani P, Pazzagli M. Developments in quantitative PCR. Clin Chem Lab Med 1998;36:255-69.
2.Chelly J, Kaplan JC, Maire P, Gautron S, Kahn A. Transcription of the dystrophin gene in human muscle and non-muscle tissue. Nature 1988;333:858-60.
3.Willey JC, Crawford EL, Jackson CM, Weaver DA, Hoban JC, Khuder SA, et al. Expression measurement of many genes simultaneously by quantitative RT-PCR using standardized mixtures of competitive templates. Am J Respir Cell Mol Biol 1998;19:6-17.
4.Crawford EL, Peters GJ, Noordhuis P, Rots MG, Vondracek M, Grafstrom RC, et al. Reproducible gene expression measurement among multiple laboratories obtained in a blinded study using standardized RT (StaRT)-PCR. Mol Diagn 2001;6:217-25.
5. DeMuth JP, Weaver DA, Crawford EL, Jackson CM, Willey JC. Loss of spr1 expression measurable by quantitative RT-PCR in human bronchogenic carcinoma cell lines. Am J Respir Cell Mol Biol 1998;19:25-9.
6.Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol 1986;51 Pt 1:263-73.
7.Rappolee DA, Mark D, Banda MJ, Werb Z. Wound macrophages express TGF-alpha and other growth factors in vivo: analysis by mRNA phenotyping. Science 1988;241:708-12.
8.Gilliland G, Perrin S, Blanchard K, Bunn HF. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci U S A 1990;87:2725-9.
9.Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-9.
10. Brooks EM, Sheflin LG, Spaulding SW. Secondary structure in the 3' UTR of EGF and the choice of reverse transcriptases affect the detection of message diversity by RT-PCR. Biotechniques 1995;19:806-12, 14-5.
11. Freeman WM, Vrana SL, Vrana KE. Use of elevated reverse transcription reaction temperatures in RT-PCR. Biotechniques 1996;20:782-3.
12. Pfeffer U, Fecarotta E, Vidali G. Efficient one-tube RT-PCR amplification of rare transcripts using short sequence-specific reverse transcription primers. Biotechniques 1995;18:204-6.
13. Ririe KM, Rasmussen RP, Wittwer CT. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 1997;245:154-60.
14. Schneeberger C, Speiser P, Kury F, Zeillinger R. Quantitative detection of reverse transcriptase-PCR products by means of a novel and sensitive DNA stain. PCR Methods Appl 1995;4:234-8.
15. Hall LL, Bicknell GR, Primrose L, Pringle JH, Shaw JA, Furness PN. Reproducibility in the quantification of mRNA levels by RT-PCR-ELISA and RT competitive-PCR-ELISA. Biotechniques 1998;24:652-8.
16. Siddiqi AM, Jennings VM, Kidd MR, Actor JK, Hunter RL. Evaluation of electrochemiluminescence- and bioluminescence-based assays for quantitating specific DNA. J Clin Lab Anal 1996;10:423-31.
17. Hayward-Lester A, Chilton BS, Underhill PA, Oefner PJ, Doris PA. Quantification of specific nucleic acids, regulated RNA processing, and genomic polymorphisms using reverse-phase HPLC, p. 45-78. In F. Ferre (Ed.), Gene Quantification. Birkhäuser, Boston. 1998;
18. Borson ND, Strausbauch MA, Wettstein PJ, Oda RP, Johnston SL, Landers JP. Direct quantitation of RNA transcripts by competitive single-tube RT-PCR and capillary electrophoresis. Biotechniques 1998;25:130-7.
19. Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci U S A 1991;88:7276-80.
20. Williams PM, Giles T, Tucker A, Winer J, Heid CA. Development and application of real-time quantitative PCR, p.313-325. In F. Ferre (Ed.), Gene Quantification. Birkhäuser, Boston. 1998.
21. Wittwer CT, Ririe KM, Rasmussen RP. Fluorescence monitoring of rapid cycle PCR for quantification, p. 129-144. In F. Ferre (Ed.), Gene Quantification. Birkhäuser, Boston. 1998.
22. Peccoud J, Jacob C. Statistical estimations of PCR amplification rates, p. 111-128. In F. Ferre (Ed.), Gene Quantification. Birkhäuser, Boston. 1998.
23. Ferre F, Marchese A, Pezzoli P, Griffin S, Buxton E, Boyer V. Quantitative PCR: an overview, p. 67-88.
In K.B. Mullis, F. Ferre and R.A. Gibbs (Eds.), The Polymerase Chain Reaction. Birkhäuser, Boston. 1994.
24. Zimmermann K, Mannhalter JW. Technical aspects of quantitative competitive PCR. Biotechniques 1996;21:268-72, 74-9.
25. Vrana SL, Kluttz BW, Vrana KE. Application of quantitative RT-PCR to the analysis of dopamine receptor mRNA levels in rat striatum. Brain Res Mol Brain Res 1995;34:127-34.
26. Becker-Andre M, Hahlbrock K. Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY). Nucleic Acids Res 1989;17:9437-46.
27. Raeymaekers L. A commentary on the practical applications of competitive PCR. Genome Res 1995;5:91-4.
28. Souaze F, Ntodou-Thome A, Tran CY, Rostene W, Forgez P. Quantitative RT-PCR: limits and accuracy. Biotechniques 1996;21:280-5.
29. Raeymaekers L. Quantitative PCR: theoretical considerations with practical implications. Anal Biochem 1993;214:582-5.

 

 

Start 1. RT-PCR 2. PCR-Detection & Monitoring 3. Competitive & Non Competitive PCR 4. PCR Protocols 5. Quantification , 6. References

 

 

RT-PCR 2010 | Privacy policy